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自1969年起,Rygaad和Povlsen将人体癌瘤移植于无胸腺小鼠以来,在耳鼻咽喉科领域内曾用鼻咽癌,舌癌,上颌窦癌,口腔底癌,喉癌等组织在无胸腺小鼠移植亦均获得成功,但用咽喉癌的转移淋巴结在无胸腺小鼠的移植,至目前为止,尚未见报道。作者们将60岁女性的咽喉癌的转移淋巴结切碎,每块为2×2×2mm~3,经处理后移植在无胸腺小鼠两侧背部。待肿瘤生长至20mm×20mm大小时,用2％副醛及2.5％Glutaradehyde灌
Since 1969, Rygaad and Povlsen have transplanted human cancers to athymic mice. In the field of otorhinolaryngology, nasopharyngeal cancer, tongue cancer, maxillary sinus cancer, oral cavity cancer, and laryngeal cancer have been used in athymic tissues. The transplantation of mice has also been successful, but transplantation of metastatic lymph nodes with throat cancer in athymic mice has not been reported so far. The authors chopped metastatic lymph nodes from 60-year-old women with throat metastases, each 2 × 2 × 2 mm × 3, and were treated and transplanted on the back of both sides of athymic mice. When the tumor grows to a size of 20mm x 20mm, it is filled with 2% paraaldehyde and 2.5% Glutaradehyde