PCR固相分析法是近年来发展起来的新技术,其主要创新是使DNA扩增产物结合在固相载体上直接进行分析和检测。与传统的凝胶电泳等法比较,本法更为简单实用。目前已在病原体的基因诊断、DNA顺序测定、扩增DNA片段的纯化及定点诱变等领域的研究中显示出良好的应用潜力。本文简要综述这一技术的基本原理及其应用。 PCR solid-phase analysis is a new technology developed in recent years. Its main innovation is to make DNA amplification products directly on the solid-phase carrier for analysis and detection. Compared with the traditional gel electrophoresis method, this method is more simple and practical. At present, it has shown good potential in the field of genetic diagnosis of pathogens, DNA sequence determination, purification of amplified DNA fragments and site-directed mutagenesis. This article briefly summarizes the basic principles of this technology and its application.