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目的探讨人类染色体端粒酶(hTERC)基因在宫颈不同上皮内病变中的扩增情况及差异性,为临床治疗宫颈疾病提供依据。方法收集液基细胞学(LCT)剩余液体121例。经活检证实,正常或良性病变30例、CIN158例、CIN218例、CIN36例、浸润性鳞癌9例。采用荧光原位杂交技术(FISH)检测宫颈脱落细胞内hTERC基因扩增情况。结果 hTERC基因在不同病例中扩增率分别为:正常或良性病变6.67%(2/30);CIN122.41%(13/58);CIN272.22%(13/18);CIN3100%(6/6);鳞癌100%(9/9)。随着病变级别的增高,TERC基因扩增率不断升高。正常及CIN1者hTERC基因扩增率显著低于CIN2及CIN2以上者(P<0.01)。CIN2以上病变hTERC基因扩增敏感度为84.85%,特异度为93.34%。结论在宫颈的不同上皮病变中都有可能发生TERC基因的扩增,且随着病变程度的加重基因扩增发生率升高。
Objective To investigate the amplification and diversity of human chromosome telomerase (hTERC) gene in different intraepithelial lesions of the cervix and provide the basis for clinical treatment of cervical diseases. Methods The remaining liquid in liquid-based cytology (LCT) was collected in 121 cases. Biopsy confirmed that normal or benign lesions in 30 cases, CIN158 cases, CIN218 cases, CIN36 cases, invasive squamous cell carcinoma in 9 cases. Fluorescence in situ hybridization (FISH) detection of exfoliated cells in cervical hTERC gene amplification. Results The amplification rates of hTERC gene in different cases were 6.67% (2/30) of normal or benign lesions, CIN122.41% (13/58), CIN272.22% (13/18), CIN3100% (6 / 6); squamous cell carcinoma 100% (9/9). As the lesion level increases, the TERC gene amplification rate continues to rise. Normal and CIN1 hTERC gene amplification rate was significantly lower than CIN2 and CIN2 (P <0.01). The sensitivity of hTERC gene amplification was 84.85% in CIN2 and the specificity was 93.34%. Conclusion TERC gene amplification may occur in different epithelial lesions of the cervix, and the incidence of gene amplification increases with the severity of the lesion.