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目的:探讨维生素A(VitA)和锌(Zn)摄入过量对幼龄大鼠脾脏抗氧化能力的影响,并为VitA和Zn的营养补充上限的制定提供实验室参考依据。方法:通过灌胃造成VitA摄入10倍于推荐摄入量(RNI)的大鼠动物模型,摄入剂量为0.48g/kg,Zn摄入20倍于RNI的大鼠动物模型,摄入剂量为1.00g/kg,并按相同剂量设立联合过量组,同时设立对照组。在实验第4周末处死全部大鼠,制备脾脏组织匀浆,测定大鼠脾脏总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性及丙二醛(MDA)含量。应用SPSS15.0统计软件对其测定结果进行统计分析。结果:实验组脾脏中T-AOC、SOD、GSH-Px、CAT活性明显降低,MDA含量显著升高,与对照组相比差异有统计学意义(P<0.05),而且VitA和Zn联合过量组较之VitA和Zn单一过量组差异更明显。结论:过量补充VitA和Zn可降低幼龄大鼠脾脏的抗氧化能力。
Objective: To investigate the effects of excessive intake of vitamin A (VitA) and zinc (Zn) on the antioxidant capacity of spleen in young rats and to provide laboratory reference for the formulation of upper limit of nutritional supplements for VitA and Zn. Methods: Rats were inoculated intragastrically with VitA up to 10 times the recommended intake (RNI) at a dose of 0.48 g / kg and Zn uptake 20 times the RNI in a rat animal model. 1.00g / kg, and the same dose set up joint overdose group, while the establishment of the control group. All the rats were sacrificed at the end of the fourth week of the experiment to prepare the spleen tissue homogenate. T-AOC, SOD, GSH- Px, CAT activity and malondialdehyde (MDA) content. SPSS15.0 statistical software for statistical analysis of its measurement results. Results: The activities of T-AOC, SOD, GSH-Px and CAT in the spleen of the experimental group were significantly lower than those in the control group (P <0.05), and the levels of MDA were significantly increased Compared with VitA and Zn single overdose difference is more obvious. Conclusion: Over-supplementation of VitA and Zn can reduce the antioxidant capacity of spleen in young rats.