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[目的]探讨不同基质金属蛋白酶在动脉硬化兔模型的动态变化及阿托伐他汀与辛伐他汀治疗的作用的比较。[方法]高脂喂食加球囊拉伤股动脉建立动脉硬化兔模型75个,分别为高脂组、辛伐他汀组和阿托伐他汀组,后两个他汀组除高脂饮食同时分别服用辛伐他汀2 mg/kg和阿托伐他汀2 mg/kg,3组均于拉伤后1、7、14、21、28 d采血,ELISA法检测MMP-1、9,TIMP-1,绘制各MMPs动态变化曲线,并取血管行病理检查。[结果]MMP-1、9在拉伤后1 d均达到峰值,延续至拉伤后7 d;TIMP-1在1 d达到峰值,并进行性降低;两个他汀组在7、14、21、28 d明显抑制MMP-1水平,辛伐他汀及阿托伐他汀组无明显区别;在21、28 d明显抑制MMP-9水平,两种他汀的作用无明显差异;而两个他汀组在1、7、14、21、28均对TIMP-1均有明显抑制,而且辛伐他汀与阿托伐他汀组之间个时间段有明显差异。[结论动脉硬化兔模型的MMPs和TIMP有不同变化规律,而相同剂量的辛伐他汀与阿托伐他汀抑制MMPs/TIMP分泌的抑制作用也不同。
[Objective] To investigate the dynamic changes of different matrix metalloproteinases in atherosclerotic rabbit models and compare the effects of atorvastatin and simvastatin. [Method] 75 rabbits with atherosclerosis were established by hyperlipidemic feeding and balloon-dwelling femoral artery. The rabbits were randomly divided into high-fat group, simvastatin group and atorvastatin group. Simvastatin 2 mg / kg and atorvastatin 2 mg / kg. The blood samples were taken at 1, 7, 14, 21 and 28 days after injury, and MMP-1 and MMP-9 and TIMP-1 were detected by ELISA Dynamic changes of each MMPs curve, and take blood line pathological examination. [Results] The levels of MMP-1 and MMP-9 reached their peak on the 1st day after injury and reached the level of 7 days after injury. The peak of TIMP-1 reached the peak on the 1st day and decreased progressively. The two statins were at 7,14,21 , 28 d significantly inhibited the level of MMP-1, simvastatin and atorvastatin group no significant difference; 21,28 d significantly inhibited the level of MMP-9, the two statins no significant difference in the role; and two statins in the 1,7,14,21,28 TIMP-1 were significantly inhibited, and simvastatin and atorvastatin group a significant difference between the time points. [Conclusion] There are different changes of MMPs and TIMP in atherosclerotic rabbit model, and the inhibitory effects of Simvastatin and atorvastatin on the secretion of MMPs / TIMP at the same dose are also different.