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目的探讨正丁酸钠对人肝癌细胞恶性表型的逆转作用。方法以0．2mmol／L的正丁酸钠（sodiumbutyrate，SB）作用于培养的人肝癌细胞GHC－3，动态观察癌细胞生长、形态、ConA凝集性、琼脂集落形成率、染色体数目、DNA合成、DNA含量等表型的变化。结果SB作用7天，人肝癌细胞生长抑制；SB作用15天ConA凝集现象减弱；作用30天后细胞形态由多角型转变为棱形，细胞ConA凝集现象基本消失；经SB作用15天、30天、45天的细胞球脂集落形成率由对照组的4．54％下降为2．53％、1.45％、1．31％；SB作用30天、45天细胞3小时3H－TdR掺入率由2330．24士10．31cpm／105cells下降为1372．5123．41cpm／105cells和1136．23土17．23cpm／105cells，细胞DNA含量由198．511．18μg/107cells下降为134．78土0．53μg/107cells和104．78土0．53μg/107cells；均有显著差异（P＜0．01）。染色体主流范围变窄，二倍体比例逐渐升高而亚三倍体比例逐渐下降，三倍体消失。结论人肝癌细胞在正了酸钠持续作用下，某些恶性表型可发生进行性逆转。
Objective To investigate the reversal effect of sodium butyrate on the malignant phenotype of human hepatoma cells. Methods 0.2 mmol/L sodium butyrate (SB) was used to treat human hepatocellular carcinoma cell line GGC-3. The growth, morphology, ConA agglutination, agar colony formation rate, chromosome number, and DNA synthesis were observed dynamically. Changes in phenotypes such as DNA content. Results After SB treatment for 7 days, the growth of human hepatocellular carcinoma cells was inhibited; ConA agglutination was weakened after SB treatment for 15 days; After 30 days, the morphology of cells changed from polygonal to prismatic, and the ConA agglutination of cells basically disappeared; SB effect was 15 days and 30 days. The 45-day cell glomerular colony formation rate decreased from 4.54% in the control group to 2.53%, 1.45%, 1.31%; SB action for 30 days and 45-day cells for 3 hours 3H-TdR incorporation rate by 2330 .24 ± 10.31 cpm/105 cells decreased to 1372.5123.41 cpm/105 cells and 1136.23 soil 17.23 cpm/105 cells. Cell DNA content decreased from 198.511.18 μg/107 cells to 134.78 ± 0.53 μg/107 cells. And 104.78 soil 0.53μg/107cells; there are significant differences (P <0.01). The mainstream of chromosomes narrowed, the proportion of diploids gradually increased and the proportion of sub-triploids gradually decreased, and triploids disappeared. Conclusion Under the sustained action of sodium sulfate, some malignant phenotypes of human hepatocellular carcinoma cells can be progressively reversed.