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目的探讨细胞色素Ｐ４５０１Ａ１（ＣＹＰ１Ａ１），２Ｄ６（ＣＹＰ２Ｄ６）和谷胱甘肽硫转移酶（ＧＳＴＭ１）基因多态性与肺癌易感性的关系。方法用病例对照研究方法及ＰＣＲ┐ＲＦＬＰ等技术检测原发性肺癌和住院对照各５９例，分析ＣＹＰ１Ａ１基因ＭｓｐＩＣ型、ＣＹＰ２Ｄ６Ｃｈ型（Ｔ／Ｔ型）和ＧＳＴＭ１缺陷型〔ＧＳＴＭ１（－）〕三种纯合突变型频率分布及其交互作用。结果突变型在病例和对照组的频率分别为（ＣＹＰ１Ａ１ＭｓｐＩＣ型２５．４％、１５．３％（Ｐ＝０．１７），ＣＹＰ２Ｄ６ＣｈＴ／Ｔ型３５．６％，４７．５％（Ｐ＝０．２６），ＧＳＴＭ１（－）型５７．６％、４９．２％（Ｐ＝０．４６），无显著性差异。协同分析发现在男性中，１１．６％（５／４３）肺癌兼有ＭｓｐＩＣ型和ＧＳＴＭ１（－）型，对照组无１例（０／４３），Ｐ＝０．０３。结论结果提示在男性中ＣＹＰ１Ａ１ＭｓｐＩＣ型和ＧＳＴＭ１（－）型可能协同增加患肺癌的危险性。
Objective To investigate the relationship between genetic polymorphisms of cytochrome P4501A1 (CYP1A1), 2D6 (CYP2D6) and glutathione S-transferase (GSTM1) and susceptibility to lung cancer. METHODS: A total of 59 cases of primary lung cancer and hospitalized controls were examined by case-control study and PCR-RFLP. Three types of CYP1A1 gene were identified: MspIC, CYP2D6Ch (T/T), and GSTM1-deficient (GSTM1(-)). Homozygous mutant frequency distribution and their interactions. Results The frequencies of the mutations in the cases and controls were (25.4% for CYP1A1 MspIC, 15.3% for P=0.17), 35.6% for CYP2D6ChT/T, and 47.5% (P=0. 26), GSTM1 (-) type 57.6%, 49.2% (P = 0.46), no significant difference. Synergistic analysis found that in men, 11.6% (5/43) lung cancer and MspIC There was no case (0/43) in the control group and GSTM1(-) type, and there was no case in the control group (P = 0.03). Conclusion The results suggest that CYP1A1 MspIC and GSTM1 (-) may synergistically increase the risk of lung cancer in men.