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目的:研究衰老过程中大鼠勃起功能变化、抗衰老蛋白SIRT1表达及其相关因子改变,探索SIRT1与老年性勃起功能障碍(ED)发生的关系。方法:SD大鼠按不同月龄分组,利用电刺激大鼠盆腔星状神经节法测定阴茎海绵体内压(ICP),颈动脉穿刺测定平均动脉压(MAP),ICP/MAP作为勃起功能的评价指标;马松染色观察胶原纤维变化;Western印迹测定不同月龄大鼠阴茎海绵体中SIRT1、P53及FOXO3a的表达变化;NO、c GMP检测试剂盒测定阴茎海绵体中NO、c GMP含量。结果:随月龄增长,大鼠勃起功能(ICP/MAP)在8月龄达到峰值,其后随月龄增长降低。c GMP含量变化与勃起功能一致。随月龄增加,大鼠阴茎海绵体中胶原纤维增多。SIRT1的表达随月龄增加不断降低;而凋亡因子P53、氧化应激因子FOXO3a的表达随月龄增加不断上升。结论:NO/c GMP通路活性降低、凋亡及氧化应激可能是衰老导致ED的原因。抗衰老蛋白SIRT1与其调控的凋亡、氧化应激因子改变与勃起功能一致,推测SIRT1与老年性ED的发病有关。
Objective: To study the changes of erectile function, the expression of anti-aging protein SIRT1 and related factors in aging rats and explore the relationship between SIRT1 and age-related erectile dysfunction (ED). Methods: The rats were divided into groups according to their age. The intraculmonary pressure (ICP) was measured by pelvic stellate ganglionometry in rats. The mean arterial pressure (MAP) measured by carotid artery puncture and ICP / MAP were evaluated as erectile function The contents of NO and c GMP in corpus cavernosum were detected by NO and c GMP detection kit. The changes of collagen fibers were observed by Matson staining. The expression of SIRT1, P53 and FOXO3a in different age groups were detected by Western blotting. Results: With the increase of age, the erectile function (ICP / MAP) peaked at 8 months and then decreased with the increase of age. c Changes in GMP content consistent with erectile function. With the increase of the age, collagen fibers in the rat penis increased. The expression of SIRT1 decreased with the increase of age, while the expression of P53 and FOXO3a increased with the increase of age. Conclusion: The activity of NO / c GMP pathway is decreased. Apoptosis and oxidative stress may be the reason of ED-induced aging. Anti-aging protein SIRT1 and its regulation of apoptosis, oxidative stress factor changes and erectile function consistent speculated that SIRT1 and the incidence of senile ED.