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目的分析寨卡病毒不同毒株分离的时间、地区以及基因型分布,基因序列的相似性,核苷酸的变异及遗传进化趋势,为寨卡的防控提供生物信息学方面的依据。方法应用Bio Edit Sequence Alignmemt Editor软件,DNAStar 7.1及Mega 7.0软件对NCBI-Nucleotide中收录的85条寨卡病毒全基因组核苷酸序列及遗传进化进行分析,探索寨卡的起源及传播过程。结果寨卡病毒组内核苷酸序列最低相似性为94.5%,最高相似性为100%;65株全基因序列中,共有765处碱基发生变异,组内变异率在0.00%~24.44%;在所有的碱基突变中,碱基转换占84.32%,而碱基颠换占15.68%;分离时间、地区相近的菌株,其遗传距离较近。结论寨卡病毒存在较高变异,这些变异可能在寨卡病毒本身的毒力、致病性及传播方式的改变中发挥重要作用。
Objective To analyze the time, region and genotype distribution of different strains of Zika virus, the similarity of gene sequences, nucleotide variation and genetic evolution trend, and provide bioinformatics basis for the prevention and control of Zika virus. Methods The nucleotide sequences and genetic evolution of 85 Zika virus genomes in NCBI-Nucleotide were analyzed by Bio Edit Sequence Alignmemt Editor software, DNAStar 7.1 and Mega 7.0 software to explore the origin and propagation of Zika virus. Results The lowest similarity among nucleotide sequences in the Zika virus group was 94.5% and the highest similarity was 100%. Among the 65 whole genome sequences, 765 bases were found in total variation, ranging from 0.00% to 24.44% In all the base mutations, the base conversion accounted for 84.32%, while the base transversion accounted for 15.68%; isolation time, the region similar strains, the genetic distance closer. Conclusion There is a high variation in Zika virus, and these variations may play an important role in the virulence, pathogenicity and changes of transmission of Zika virus itself.