Study on spectroscopy of two flavonyl glycosides from Andrographis panlculata

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To study the bioactive constituents from Andrographis paniculata,two compounds were isolated and purified by column chromatography on AB-8 macroporous adsorption resin and polyide.Their structures were determined by various spectroscopic methods,including UV,FABMS,1H-NMR,13C-NMR,DQFCOSY,TOCSY,HMQC,HMBC,and NOESY.The 1H-NMR and 13C-NMR signals of the two compounds were assigned.The structures of the two compounds were elucidated as 5,4’-dihydroxy-7-methoxyflavone-6-yl-β-D-glucopyranoside and 5,4’-dihydroxy-7-methoxyflavone- 8-yl-β-D-glucopyranoside,respectively.
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