邮购的银耳木屑原种,在长途运输中,由于受到外界温度、湿度和通风等环境条件变化的影响,易使杂菌侵入瓶内。如果直接用于栽培生产,容易造成杂菌孳生而失败。所以,必须采取破瓶法接菌。具体操作如下: 把经过灭菌的培养基菌种瓶(或空瓶)和接菌工具等放进接菌箱,将邮购的菌种瓶外用75％酒精擦洗二遍,放进箱内,用高锰酸钾和福尔马林气化密封消毒30～40分钟(用量每m~3用高锰酸钾5克、福尔马林10毫升)。操作者戴橡胶手套,并用75％酒精或 Mail order Tremella sawdust original species, long-distance transport, due to external temperature, humidity and ventilation and other environmental conditions change, easy to make bacteria invade the bottle. If used directly for cultivation and production, easy to cause miscellaneous bacteria breeding and failure. Therefore, we must take the broken bottle law bacteria. Specific operations are as follows: The sterilized culture flask (or empty bottle) and bacteria inoculation tools into the inoculation box, mail order bacteria outside the bottle scrub with 75% alcohol two times, into the box with Potassium permanganate and formalin gasification sealed 30 to 40 minutes (dosage of potassium permanganate 5 grams, formalin 10 ml). Operators wear rubber gloves and use 75% alcohol or