An organically templated 2-D uranyl sulfate, {(C2H8N)[(UO2)Cl(SO4)(H2O)]}n 1, has been hydrothermally synthesized . The crystal and molecular structures have been determined by X-ray crystallography method and spectral techniques. 1 belongs to mono- clinic, space group P21/c with a = 8.3545(17), b = 10.550(2), c = 12.370(3)(A), β = 102.64(3)°, V = 1063.9(4)(A)3, Mr = 464.64, Dc = 2.901 g/cm3, F(000) = 836, μ = 15.710 mm-1, Z = 4, the final R = 0.0286 and wR = 0.0685 for 10164 observed reflections with I > 2σ(I). 1 presents a two-dimensional layer-like structure constructed from infinite anionic [(UO2)Cl(H2O)(SO4)]- layers with [C2H8N]+ cations balancing the charge and a number of intermolecular hydrogen bonds (C-H…O and O-H…Cl) existing in the solid state. The fluorescence properties of 1 have also been discussed.