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在缺血性脑损伤的发病机理和防治的实验研究中,缺少显示缺血区和定量测定的方法,难以分析实验结果及客观评价药物等防治措施。作者建立的NBT染色法,能较好地显示脑缺血区,并用计算机定量客观的测定,是脑血管病实验研究的一个有效手段。 以手术阻断大鼠一侧大脑中动脉24～48h后,将动物断头,快速剥出全脑,低温下作脑冠状切片,每片厚约2mm,37℃ NBT染色液中温育30min,正常脑组织呈现均匀的蓝紫色,脑灰质着色较白质稍深,
In the experimental study on the pathogenesis and prevention of ischemic brain injury, there is a lack of methods to display the ischemic area and quantitative determination, it is difficult to analyze the experimental results and objective evaluation of drugs and other prevention and treatment measures. The NBT staining method established by the author can better display the cerebral ischemic area and make a quantitative and objective determination by computer. It is an effective means for the experimental study of cerebrovascular disease. After surgery, the animals were decapitated and the entire brain was quickly stripped. The brains were sliced at low temperature for 2 mm. Each piece was incubated in NBT staining solution at 37 ° C for 30 min. Normal Brain tissue showed a uniform blue-purple, gray matter coloring slightly darker than the white matter,