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在10℃黑暗条件下进行室内土培接种实验,评价玉米秸秆低温降解复合菌系GF-20对玉米秸秆的促分解作用及对土壤微生物群落多样性的影响。在接种后15、30、45和60 d分别测定GF-20及对照菌剂LK和SM的玉米秸秆降解率及土壤微生物群落结构。结果表明,与未施菌剂对照(CK)相比,施用菌剂在一定程度上加快玉米秸秆的分解,以低温高效菌剂GF-20效果最佳,可明显加快秸秆分解进程。利用16Sr DNA-PCR-DGGE技术检测土壤微生物群落的多样性,与未施菌剂对照(CK)和无秸秆无菌剂的空白对照(B)相比,施用菌剂对土壤细菌群落结构产生较大的影响,可显著提高土壤细菌富度(S)和多样性指数(H),表现为GF-20>LK>SM>CK>B;各处理之间的种群均匀度指数(E)差异不显著。玉米秸秆添加促腐解菌剂,可有效加快秸秆分解,增加土壤微生物群落多样性。
Soil culture inoculation experiments were conducted at 10 ℃ in darkness to evaluate the effects of low temperature degradation of corn stalk composite strain GF-20 on the decomposition of corn stalks and the diversity of soil microbial communities. At 15, 30, 45 and 60 days after inoculation, the degradation rates of corn stalks and the microbial community structure of GF-20 and control fungi LK and SM were determined. The results showed that, compared with the control (CK), the fungicide application accelerated the decomposition of corn stalk to a certain degree, and the effect of low temperature and high efficient fungi agent GF-20 was the best, which could obviously accelerate the process of straw decomposition. The 16Sr DNA-PCR-DGGE was used to detect the diversity of soil microbial community. Compared with CK (CK) and blank control without straw (B) (S) and diversity index (H) of soil were significantly increased, showing GF-20> LK> SM> CK> B. Differences in population evenness index (E) Significant. The addition of corn stalk to promote decay agent can effectively accelerate the decomposition of straw and increase the diversity of soil microbial community.