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[目的]探究甲醛和三氯乙烯联合染毒对小鼠肾脏的氧化损伤作用。[方法]共108只清洁级昆明种小鼠,按3×3析因设计随机平均分为9组:对照组(清洁空气)、甲醛低(1 mg/m3)、甲醛高(5 mg/m3)、三氯乙烯低(1 000 mg/m3)、三氯乙烯高(5 000 mg/m3)、甲醛低+三氯乙烯低(1 mg/m3+1 000 mg/m3)、甲醛低+三氯乙烯高(1 mg/m3+5 000 mg/m3)、甲醛高+三氯乙烯低(5 mg/m3+1 000 mg/m3)、甲醛高+三氯乙烯高(5 mg/m3+5 000 mg/m3)剂量组,每组12只,雌雄各半。采用静式吸入染毒,每天2 h,连续14 d。染毒结束后测定肾组织总抗氧化能力(total antioxidant capacity,T-AOC)、还原型谷胱甘肽(glutathione,GSH)、总超氧化物歧化酶(superoxide dismutase,SOD)和丙二醛(malondialdehyde,MDA)含量。[结果]小鼠肾组织T-AOC、SOD和GSH随染毒剂量升高而明显降低,MDA含量则明显上升,差异均有统计学意义(P<0.05)。其中,联合染毒对雌性小鼠T-AOC的影响存在一定交互作用,具有统计学意义(P<0.05),其他各指标的交互作用并不显著。[结论]甲醛和三氯乙烯吸入染毒对小鼠肾脏均具有氧化损伤作用,两者联合可能存在一定的交互作用。
[Objective] To explore the oxidative damage of mouse kidney induced by the combination of formaldehyde and trichlorethylene. [Method] A total of 108 Kunming mice of clean grade were randomly divided into 9 groups according to 3 × 3 factorial design. The control group (clean air), low formaldehyde (1 mg / m3), high formaldehyde (1 000 mg / m3), trichlorethylene high (5 000 mg / m3), formaldehyde low + trichlorethylene low (1 mg / m3 + 1 000 mg / m3) (1 mg / m3 + 5 000 mg / m3), high formaldehyde + trichlorethylene (5 mg / m3 + 1 000 mg / m3), high formaldehyde + trichlorethylene (5 mg / m3 +5 000 mg / m3) dose group, each group of 12, male and female half. Acute inhalation exposure, 2 h daily for 14 days. The total antioxidant capacity (T-AOC), glutathione (GSH), total superoxide dismutase (SOD) and malondialdehyde (MDA) malondialdehyde, MDA) content. [Result] The T-AOC, SOD and GSH in the kidney of mice were significantly decreased with the increase of the dose, but the content of MDA was significantly increased (all P <0.05). Among them, the effect of joint exposure on T-AOC in female mice had some interaction, with statistical significance (P <0.05). The interaction of other indexes was not significant. [Conclusion] The inhalation of formaldehyde and trichlorethylene have oxidative damage on the kidneys of mice, and there may be some interaction between the two.