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目的 :对以左旋聚乳酸(poly L-lactic acid,PLLA)及聚乙烯醇(polyvinyl alcohol,PVA)为膜材的微气泡,在稳定性和粒径两个方面进行了优化,使其在保持超声成像增强能力的同时,更适于体内应用。方法:首先对PVA进行了羧基化修饰,使制备的微气泡表面含有羧基,Zeta电位达到-38.87±2.19 m V,从而由于静电斥力提高了微气泡的稳定性。其次,通过调节制备过程中的PVA浓度、水相油相比、乳化速度3种因素,实现了对微气泡粒径的控制。结果:对优化后平均粒径为1.38μm、粒径分布均匀、性质稳定的微气泡进行了体内外超声成像实验,证实其在体外能够显著增强超声成像效果,在体内既可以通过瘤内注射迅速提高肿瘤部位超声显影质量,又可以通过静脉注射,经血液循环到达肿瘤部位,在一段时间的累积之后,增强超声成像效果。结论:本研究为微气泡的体内应用打下了良好基础。
OBJECTIVE: To optimize the stability and particle size of microbubbles with poly-L-lactic acid (PLLA) and polyvinyl alcohol (PVA) as membrane material, Ultrasound imaging while enhancing the ability of more suitable for in vivo applications. Methods: The PVA was modified by carboxylation to make the surface of the prepared microbubbles contain carboxyl groups and the Zeta potential reached -38.87 ± 2.19 mV, and the stability of microbubbles was enhanced by electrostatic repulsion. Secondly, the control of the particle size of the microbubbles is achieved by adjusting three factors of the concentration of PVA, the oil phase in the oil phase and the emulsifying speed in the preparation process. Results: Ultrasound imaging of microbubbles with an average particle diameter of 1.38μm, uniform particle size distribution and stable properties was performed in vitro and in vivo. The results showed that in vitro ultrasound imaging can significantly enhance the imaging effect and both in vivo and in vivo Improve the quality of ultrasound imaging of the tumor site, but also by intravenous injection, blood circulation to the tumor site, after a period of accumulation, enhanced ultrasound imaging. Conclusion: This study laid a good foundation for in vivo application of microbubbles.