Zika virus (ZIKV) infection could disrupt neurogenesis and cause microcephaly in neonates by targeting neural progenitor cells (NPCs).The tumor suppressor p53-mediated cell cycle arrest and apoptotic cell death have been suggested to be activated upon ZIKV infection,yet the detailed mechanism is not well understood.In the present study,we investigated the effects of ZIKV-encoded proteins in the activation of p53 signaling pathway and found that,among the ten viral proteins,the nonstructural protein 5 (NS5) of ZIKV most significantly activated the transcription of p53 target genes.Using the immunoprecipitation-coupled mass spectrometry approach,we identified that ZIKV-NS5 interacted with p53 protein.The NS5-p53 interaction was further confirmed by co-immunoprecipitation and GST pull-down assays.In addition,the MTase domain of NS5 and the C-terminal domain of p53 were mapped to be responsible for the interaction between these two proteins.We further showed that ZIKV-NS5 was colocalized with p53 and increased its protein level in the nuclei and able to prolong the half-life of p53.Furthermore,lentivirus-mediated expression of ZIKV-NS5 in hNPCs led to an apparent cell death phenotype.ZIKV-NS5 promoted the cleavage of PARP1 and significantly increased the cell apoptosis of hNPCs.Taken together,these findings revealed that ZIKV-NS5 is a previously undiscovered regulator of p53-mediated apoptosis in hNPCs,which may contribute to the ZIKV-caused abnormal neurodevelopment.