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目的:采用高效液相色谱法测定溃疡宁片中甘草苷及甘草酸的含量。方法:色谱柱:Agilent HC-C_(18)(250mm×4.6mm,5μm);流动相:以乙腈为流动相A,0.1%磷酸为流动相B,按一定比例进行梯度洗脱;检测波长为237nm。结果:甘草苷进样量在0.089～0.447μg范围内峰面积积分值与进样量呈良好的线性关系(r=0.999 7),平均加样回收率99.54%,RSD=0.20%(n=6);甘草酸进样量在0.516～2.582μg范围内峰面积积分值与进样量呈良好的线性关系(r=0.9999),平均加样回收率100.34%,RSD=0.41%(n=6)。结论:该方法易于操作,结果准确,重复性好,可用于溃疡宁的质量控制。
Objective: To determine the content of glycyrrhizin and glycyrrhizin in Kuiyining Tablet by HPLC. The mobile phase consisted of acetonitrile as mobile phase A and 0.1% phosphoric acid as mobile phase B, and the gradient elution was carried out at a certain ratio. The detection wavelength was 237nm. Results: The peak area integral value of liquiritin in the range of 0.089 ~ 0.447μg showed a good linear relationship with the injection volume (r = 0.999 7), the average recovery was 99.54%, RSD was 0.20% (n = 6 ). The peak area integral value of glycyrrhizic acid in the range of 0.516 ~ 2.582μg showed a good linear relationship with the injection volume (r = 0.9999), the average recovery was 100.34%, RSD was 0.41% (n = 6) . Conclusion: The method is easy to operate with accurate results and good reproducibility. It can be used for the quality control of ulcer Ning.