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收集麻疹病人急性期的尿液和咽拭子标本 ,比较这两种临床标本在B95a细胞中麻疹病毒分离的阳性率 ,为今后分离病毒所用标本的收集提供指导 ,为我国麻疹病毒流行毒株基因型的鉴定提供更多的分离株。采用的方法是以B95a细胞分离麻疹病毒 ,对标本的运送、储存条件及处理方法进行标准化。结果显示 :尿标本麻疹病毒分离率为 4 1 17% ,比咽拭子高 3 5倍。初步提示 ,为分离麻疹病毒 ,最好在出疹 1～ 3d采集标本 ,而且以采集尿标本为好
Urine and throat swab specimens were collected during the acute phase of measles and the positive rates of measles virus isolation in B95a cells were compared to provide guidance for the collection of specimens for virus isolation in the future. Type identification provides more isolates. The method used is to isolate the measles virus in B95a cells and to standardize the shipping, storage and handling of specimens. The results showed that the urine sample measles virus isolation rate was 41.17%, 35 times higher than the throat swab. Preliminary tips, for the separation of measles virus, the best rash 1 ~ 3d specimens collected, but also to collect urine specimens as well