【摘 要】
：
目的构建包含FMDV结构蛋白P1、非结构蛋白2A、3C以及部分2B的基因片段P12X3C的植物组成型表达载体,为FMDV可饲疫苗的研制奠定基础.方法通过PCR扩增法,从pGEM/P12A质粒和pGEM/
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目的构建包含FMDV结构蛋白P1、非结构蛋白2A、3C以及部分2B的基因片段P12X3C的植物组成型表达载体,为FMDV可饲疫苗的研制奠定基础.方法通过PCR扩增法,从pGEM/P12A质粒和pGEM/3C质粒中扩增P12A及部分2B基因(P12X)、 3C基因,将获得的P12X与3C片段经BamHI/SpeI和SpeI/SalI双酶切后,与经BamHI/SalI双酶切后的pUC18质粒大片段连接,得到重组质粒pUC18/P12X3C,pUC18/P12X3C质粒经BamHI/SalI双酶切后的大片段与经同样双酶切的植物表达载体pBin438连接,转化子经酶切、PCR及测序鉴定后,获得包含FMDV O/China/99株P12X3C片段的植物组成型表达载体pBin438/P12X3C,最后通过三亲交配法,将表达载体pBin438/P12X3C导入农杆菌.结论成功构建FMDV多基因的植物组成型表达载体.
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