【摘 要】
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Ammonia induces astrocyte swelling, which is strongly associated with overexpression of aquaporin-4.However, the mechanisms by which ammonia induces astrocyte s
【机 构】
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Department of Anesthesiology
【基金项目】
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Zhejiang Provincial Health Department
论文部分内容阅读
Ammonia induces astrocyte swelling, which is strongly associated with overexpression of aquaporin-4.However, the mechanisms by which ammonia induces astrocyte swelling, and subsequently upregulating aquaporin-4 expression, remain unknown.In the present study,astrocytes were cultured in vitro and exposed to ammonium chloride (NH4CI), followed by propofol,protein kinase C agonist, or antagonist, respectively.Astrocyte morphology was observed by light microscopy, and aquaporin-4 expression was detected by western blot analysis.Results showed that propofol or protein kinase C agonist significantly attenuated the degree of NH4CI-induced astrocyte swelling and inhibited increased aquaporin-4 expression.Propofol treatment inhibited aquaporin-4 overexpression in cultured astrocyte induced by NH4CI; protein kinase C pathway activation is potentially involved.
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