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目的探讨丹参酮对异丙肾上腺素诱导的心肌细胞肥大的影响。方法采用皮下注射异丙肾上腺素造成心肌肥厚大鼠模型,通过免疫组织化学方法观察丹参酮对肥大心肌细胞凋亡相关因子Fas、Bcl-2表达的影响。结果异丙肾上腺素组大鼠心肌肥厚,Fas、Bcl-2基因表达增加(P<0.05),加入丹参酮干预后,Fas基因表达减弱(P<0.05),Bcl-2基因表达增加(P<0.05)。结论丹参酮具有保护心肌,预防心肌肥厚的作用,其机制可能与抑制Fas基因表达,增加Bcl-2基因表达有关。
Objective To investigate the effect of tanshinone on isoprenaline-induced cardiomyocyte hypertrophy. Methods A rat model of myocardial hypertrophy was induced by subcutaneous injection of isoproterenol. The effect of tanshinone on the expression of apoptosis-related factors Fas and Bcl-2 in hypertrophic cardiomyocytes was observed by immunohistochemistry. Results The myocardial hypertrophy, Fas and Bcl-2 gene expression were increased in isoproterenol group (P<0.05). After intervention with tanshinone, the expression of Fas gene was attenuated (P<0.05), and the expression of Bcl-2 gene was increased (P<0.05). ). Conclusion Tanshinone can protect myocardium and prevent myocardial hypertrophy. The mechanism may be related to inhibiting the expression of Fas gene and increasing the expression of Bcl-2 gene.