【摘 要】
:
目的 建立高效液相色谱法测定女贞子中特女贞苷的含量的方法.方法 采用Alhima C18色谱柱(4.6mm×250mm,5 μm),乙腈-水为流动相,流速1.0 ml/min,检测波长230 nm,柱温25℃.在
【机 构】
:
广东广州,广州中医药大学第一附属医院
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目的 建立高效液相色谱法测定女贞子中特女贞苷的含量的方法.方法 采用Alhima C18色谱柱(4.6mm×250mm,5 μm),乙腈-水为流动相,流速1.0 ml/min,检测波长230 nm,柱温25℃.在此色谱条件下,对女贞子中特女贞苷含量进行测定.结果 该方法在0.05-0.4mg/ml范围内线性关系良好,回收率为99.27%,RSD为0.87%,女贞子中特女贞苷的平均含量为9.39 mg/g.结论 该方法简便、准确,重复性好,可用于控制女贞子的质量.
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