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目的探讨组蛋白甲基化酶SMYD3在前列腺癌组织的表达水平及其对前列腺癌细胞生长的影响。方法免疫组化染色检测33例前列腺癌及癌旁组织的SMYD3表达水平;siRNA及SMYD3过表达质粒分别转染前列腺癌LNCa P和PC3细胞,检测其对前列腺癌细胞系生长的影响;Western blotting检测前列腺癌细胞中mTOR通路相关蛋白表达水平。结果 SMYD3在前列腺癌组织的表达水平明显高于癌旁组织,且SMYD3在胞核胞浆中均可检测到;转染SMYD3 siRNA后处理组LNCa P细胞在不同生长时期均少于对照组;而PC3细胞在转染SMYD3过表达质粒后细胞数则多于对照组。处理后,LNCa P细胞p-mTOR、p-p70S6K基因表达降低(P<0.05),而p-4EBP-1蛋白表达上升(P<0.05);PC3细胞呈现一致趋势。结论前列腺癌组织中SMYD3表达水平高于癌旁组织,且在胞核胞浆中均有表达;SMYD3表达可以促进前列腺癌细胞的生长;且可通过激活mTOR基因,参与mTOR通路的调节,影响前列腺癌细胞系的生长。
Objective To investigate the expression of histone methyltransferase SMYD3 in prostate cancer and its effect on the growth of prostate cancer cells. Methods Immunohistochemical staining was used to detect the expression of SMYD3 in 33 cases of prostate cancer and its adjacent tissues. The siRNA and SMYD3 overexpression plasmids were transfected into prostate cancer LNCa P and PC3 cells, respectively. The effect of SMYD3 on the growth of prostate cancer cell lines was detected by Western blotting Prostate cancer cells mTOR pathway-related protein expression levels. Results The expression level of SMYD3 in prostate cancer tissues was significantly higher than that in paracancerous tissues, and SMYD3 was detected in cytoplasm of cytoplasm. LNCa P cells transfected with SMYD3 siRNA were less than those in control group at different growth stages; PC3 cells transfected with SMYD3 overexpression plasmid after the cell number is more than the control group. After treatment, the expression of p-mTOR and p-p70S6K genes in LNCa P cells decreased (P <0.05), while the expression of p-4EBP-1 protein increased (P <0.05). PC3 cells showed a consistent trend. Conclusions The expression of SMYD3 in prostate cancer tissues is higher than that in paracancerous tissues and is expressed in cytoplasm of nucleus. SMYD3 expression can promote the growth of prostate cancer cells. It can also affect the regulation of mTOR pathway by activating mTOR gene Growth of cancer cell lines.