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目的: 建立同时测定金衣万应丸中儿茶素、表儿茶素、胡黄连苷Ⅲ、胡黄连苷Ⅱ和胡黄连苷Ⅰ的HPLC 波长切换法.方法: 选用Agilent TC-C18(250 mm×4. 6 mm,5 μm)色谱柱; 流动相: 乙腈-0. 2％磷酸溶液(梯度洗脱); 流速0. 9 mL/min; 柱温30 ℃; 进样量20 μL.结果: 金衣万应丸中儿茶素、表儿茶素、胡黄连苷Ⅲ、胡黄连苷Ⅱ和胡黄连苷Ⅰ分别在6. 12 ～ 122. 40、3. 78 ～ 75. 60、 3. 35～67. 00、12. 76 ～ 255. 20、8. 94 ～ 178. 80 μg /mL 浓度范围内线性关系良好,平均回收率(n = 6)分别为98. 40％(RSD = 1. 71％)、97. 56％(RSD= 1. 24％)、96. 94％(RSD = 0. 88％)、100. 19％(RSD = 0. 99％)、98. 97％(RSD = 1. 03％).结论: 该方法简便、准确、重复性好,可为金衣万应丸多指标质量评价提供参考.“,”Objective: To establish the quantitative method of (+ ) -catechin, epicatechin, picroside Ⅲ, amphicoside Ⅱ and amphicosideⅠ in Jinyi Wanying pills by HPLC wavelength switching method. Methods: The determination was performed on chromatographic column of Agilent TC-C18(250 mm × 4. 6 mm, 5 μm). The mobile phase consisted of acetonitrile (A)-0. 2％ of phosphoric acid solution (B)with gradient elution. The flow rate was 0. 9 mL/min. The column temperature was set at 30℃. The injection volume was 20 μL. Results: The concentration ranges of (+)-catechin, epicatechin, picroside Ⅲ, amphicoside Ⅱ and amphicosideⅠ were respectively 6. 12-122. 40, 3. 78-75. 60, 3. 35-67. 00, 12. 76-255. 20, 8. 94-178. 80 μg /mL, with well linear relation. The average recovery of the above five components were 98. 40％ (RSD=1. 71％), 97. 56％ (RSD=1. 24％), 96. 94％ (RSD=0. 88％), 100. 19％ (RSD=0. 99％), 98. 97％ (RSD=1. 03％), respectively. Conclusion: This determination method is convenient and accurate, with good repeatability, which can provide references for multi-indices quality evaluation of Jinyi Wanying pills.