论文部分内容阅读
将１５例外科手术切下肺癌组织和同源正常肺组织碱性磷酸酶（ＡＬＰ）进行比较。在１１例癌组织中，ＡＬＰ酶活性显著低于同源正常肺组织。其余４例，两者酶活性无差异。癌组织与同源肺组织ＡＬＰ的热稳定性，电泳行为以及对Ｌ－Ｐｈｅ和尿素抑制的敏感性，均未发现不同，但不同于胎盘ＡＬＰ。显示两者均属于组织非特异性ＡＬＰ同工酶。实验提示，肺癌中，组织非特异性ＡＬＰ活性降低。这可能系肿瘤中存在该酶的抑制剂或在基因转录水平异常所致。
Fifteen surgically resected lung cancer tissues and homologous normal lung tissue were compared for alkaline phosphatase (ALP). In 11 cases of cancerous tissue, ALP enzyme activity was significantly lower than homologous normal lung tissue. In the remaining 4 cases, there was no difference in enzyme activity between the two groups. The thermal stability, electrophoretic behavior, and sensitivity to L-Phe and urea inhibition of ALP in cancer tissue and homologous lung tissue were not different, but were different from placental ALP. Both are shown to belong to tissue non-specific ALP isoenzymes. Experiments have shown that nonspecific ALP activity in tissues is reduced in lung cancer. This may be due to the presence of an inhibitor of the enzyme in the tumor or due to abnormal levels of gene transcription.