目的观察乳腺浸润性导管癌组织和癌旁正常乳腺组织中脂质代谢调控基因的表达,并探讨其与乳腺浸润性导管癌发生的关系。方法收集30例乳腺浸润性导管癌患者手术切除的乳腺癌组织和癌旁乳腺组织,RT-PCR检测各组织中脂肪酸合成酶(fatty acid synthase,FAS)、环氧合酶(cyclooxygenase-2,Cox-2)、5-脂氧合酶(5-lipoxygenase,5-LOX)及过氧化物酶增殖活化受体γ(peroxisome proliferator-activated receptorγ,PPAR-γ)的基因表达。结果乳腺浸润性导管癌组织中FASmRNA的表达显著高于癌旁组织(3.265±0.913vs1.300±0.712,P<0.01),同时乳腺癌组织中Cox-2、5-LOXmRNA的表达显著高于癌旁组织(2.449±1.333vs1.027±0.596,P<0.05;2.766±1.193vs1.159±0.520,P<0.05),PPAR-γmRNA的表达在乳腺癌组织和癌旁乳腺组织间差异无统计学意义(2.771±1.313vs2.235±1.383,P>0.05)。结论 FAS、Cox-2及5-LOX基因的高表达与乳腺浸润性导管癌的发生有关,提示脂质代谢途径调控对乳腺癌的预防具有重要的意义。 Objective To observe the expression of lipid metabolism regulatory genes in infiltrating ductal carcinoma tissues and adjacent normal breast tissues and to investigate their relationship with the infiltrating ductal carcinomas. Methods Thirty specimens of breast cancer tissues and paracancer tissues were collected from patients with breast invasive ductal carcinoma. The expressions of fatty acid synthase (FAS), cyclooxygenase-2 (Cox) -2), 5-lipoxygenase (5-LOX) and peroxisome proliferator-activated receptorγ (PPAR-γ). Results The expression of FAS mRNA in breast invasive ductal carcinoma tissues was significantly higher than that in paracancer tissues (3.265 ± 0.913 vs 1.300 ± 0.712, P <0.01), and the expression of Cox-2,5-LOX mRNA in breast cancer tissues was significantly higher than that in cancer tissues (2.449 ± 1.333vs1.027 ± 0.596, P <0.05; 2.766 ± 1.193vs.1.159 ± 0.520, P <0.05). There was no significant difference in the expression of PPAR-γmRNA between breast cancer tissues and adjacent normal breast tissues (2.771 ± 1.313 vs 2.235 ± 1.383, P> 0.05). Conclusion The high expression of FAS, Cox-2 and 5-LOX genes is related to the occurrence of breast invasive ductal carcinomas, suggesting that the regulation of lipid metabolism is of great significance for the prevention of breast cancer.