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目的:监测2008年温州市EV71感染情况,建立温州市手足口病EV 71型检测系统,预防和控制温州市EV71的大规模流行。方法:将样本中病毒进行核酸提取,通过荧光定量PCR、RT-PCR扩增及其电泳,分别检测肠道病毒通用型和EV71型、Cox A16;对EV71型病毒进行VP1测序和亚型分析。结果:检测284份标本的核酸样品,有179份样品为肠道通用病毒阳性,85份样品EV71阳性,4份样品CoxA16阳性。结论:温州市EV71感染主要集中在沿海区域,春夏季高发,易感人群为5岁以下儿童,主要流行的基因型为C4亚型。
OBJECTIVE: To monitor EV71 infection in Wenzhou in 2008 and establish Wenzhou EV71 detection system to prevent and control the large-scale epidemic of EV71 in Wenzhou City. Methods: The nucleic acid of the virus was extracted from the samples. The total enterovirus and EV71 and Cox A16 were detected by real-time PCR, RT-PCR and electrophoresis respectively. The VP1 of EV71 was sequenced and the subtype was analyzed. RESULTS: Of the 284 samples tested for nucleic acid, 179 were positive for enteric virus, 85 were positive for EV71 and 4 were positive for CoxA16. Conclusion: The EV71 infection in Wenzhou City is mainly concentrated in the coastal areas, with high incidence in spring and summer. The susceptible population is children under 5 years old. The main epidemic genotype is C4 subtype.