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本实验采用雄性Wistar大鼠,体重60～80 g,在无菌条件下打开腹腔,将少量冰醋酸注射入胃粘膜下层,造成胃溃疡。手术后10天,大鼠重80～100 g,以50 μCi/100 g~(125)I-T_3的放射性剂量(比放射性为3,000 μCi/μg)由颈静脉直接注射入体内。注射120分钟后做以下处理:1.取胃溃疡局部组织,入10％中性福尔马林液固定,石蜡切片(厚5μm),脱蜡,涂核乳胶,4℃曝光5～10天;2.用2％的戊二醛液(0.1 mol/L磷酸缓冲液配制,pH
In this study, male Wistar rats weighing 60-80 g were used to open the abdominal cavity under aseptic conditions. A small amount of glacial acetic acid was injected into the gastric mucosa to cause gastric ulcer. Ten days after the operation, the rats weighed 80-100 g and were directly injected into the body from the jugular vein at a radioactive dose of 50 μCi / 100 g to 125 T-3 (specific radioactivity of 3,000 μCi / μg). After 120 minutes of injection, the following treatments were performed: 1. The local tissue of gastric ulcer was taken, fixed in 10% neutral formalin, paraffin section (thickness 5μm), dewaxed and coated with latex, 2. With 2% glutaraldehyde solution (0.1 mol / L phosphate buffer formulated, pH