等电聚焦电泳是分离蛋白质(同工酶)最有效的方法之一。本文以杂交水稻特优524(F_1)及其父本、母本的种子为材料,对它们胚芽时期的酯酶等同工酶进行等电聚焦电泳分析。结果表明:特优524(F_1)的酯酶同工酶有4条酶带,其父本有2条酶带,其母本有3条酶带,混杂种子有5条酶带。测出此杂交水稻种子的纯度为94.6%,与田间鉴定相符合。 Isoelectric focusing electrophoresis is one of the most effective ways to isolate proteins (isozymes). In this study, the isozymes of esterase isozymes in hybrid rice Teyou 524 (F_1) and their male and female parents were analyzed by isoelectric focusing. The results showed that there were 4 bands in esterase isozyme of Teyou 524 (F_1), 2 bands in the male parent, 3 bands in the female parent and 5 bands in the mixed seed. The purity of this hybrid rice was 94.6%, which was consistent with the field identification.