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目的：探讨nm23、p21和c-erbB-2基因在中期胃癌的表达及其临床意义。方法：应用免疫组化(SP)方法，检测手术切除标本癌组织中nm23、p21和c-erbB-2的表达。结果：(1)69例中期胃癌标本中的nm23低表达、p21及c-erbB-2过表达率分别为71.0％(49/69)、60.9％(42/69)及66.7％(46/69);(2)无淋巴结转移及Ⅰ＋Ⅱ期胃癌的nm23表达率(87.5％及83.3％)分别高于有淋巴结转移及Ⅲ＋Ⅳ期病例的表达率(62.2％及57.6％)(P<0.05及P<0.05);(3)肠型胃癌的p21表达率(42.9％)及cerbB2表达率(35.7％)分别低于弥漫型病例的表达(73.2％及87.8％)(P<0.01及P<0.01)，Ⅰ＋Ⅱ期胃癌p21表达率(33.3％)及c-erbB-2表达率(44.4％)显著低于Ⅲ＋Ⅳ期病例的表达(90.9％及90.9％)(P<0.01及P<0.01)；(4)nm23、p21和c-erbB-2两个以上联合表达病例与表达阴性或单因素表达者相比，在胃癌的组织学类型、浸润方式、脉管浸润、淋巴结转移及临床分期方面差异显著(P<0.05或P<0.01)。结论：中期胃癌组织中nm23、p21和c-erbB-2表达与淋巴结转移、组织学类型及临床分期密切相关,提示这些基因在胃癌细胞的增殖过程中可能起重要作用。
Objective: To investigate the expression and clinical significance of nm23, p21 and c-erbB-2 genes in metaphase gastric cancer. Methods: The expression of nm23, p21 and c-erbB-2 was detected by immunohistochemistry (SP) method in surgical specimens. Results: (1) The low expression of nm23, overexpression of p21 and c-erbB-2 in 69 cases of metastatic gastric cancer were 71.0% (49/69), 60.9% (42/69) and 66.7% (46/69), respectively. ); (2) The rate of nm23 expression in lymph node metastasis and stage I+II gastric cancer (87.5% and 83.3%) was higher than that in lymph node metastasis and stage III+IV (62.2% and 57.6%), respectively (P<0.05 and P<0.05). 0.05); (3) The expression rate of p21 (42.9%) and cerbB2 (35.7%) in intestinal type gastric cancer were lower than those in diffuse type (73.2% and 87.8%, respectively) (P<0.01 and P<0.01). The expression rate of p21 (33.3%) and c-erbB-2 (44.4%) in stage I+II gastric cancer were significantly lower than those in stage III+IV (90.9% and 90.9%) (P<0.01 and P<0.01); (4) Two or more cases of combined expression of nm23, p21, and c-erbB-2 were significantly different from those with negative or univariate expression in terms of histological type, infiltration pattern, vascular invasion, lymph node metastasis, and clinical staging. <0.05 or P<0.01). CONCLUSIONS: The expression of nm23, p21, and c-erbB-2 in metastatic gastric cancer is closely related to lymph node metastasis, histological type, and clinical stage, suggesting that these genes may play an important role in the proliferation of gastric cancer cells.