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目的检测高砷煤引起的砷中毒患者外周血红细胞血型糖蛋白A(GPA)的突变频率。方法分离、固定40例高砷煤引起的砷中毒患者外周血红细胞,与荧光素标记的单抗结合后,采用流式细胞仪进行GPA突变频率分析。结果40例高砷煤引起的砷中毒患者外周血红细胞GPA突变频率:NN突变频率为(21.23±13.97)×10-6,NO突变频率为(33.13±25.72)×10-6,MM突变频率为(110.90±63.58)×10-6,MO突变频率为(20.35±21.26)×10-6,GPA的突变频率明显高于正常人群(P <0.01)。肿瘤组:NN突变频率为(31.50±16.13)×10-6,NO突变频率为(54.50±38.13)×10-6,MM突变频率为(159.33±66.22)×10-6,MO突变频率为(45.16±12.69)×10-6,GPA的突变频率明显高于非肿瘤组(P<0.01)。结论砷中毒可导致人类外周血红细胞GPA的突变。提示GPA的突变频率值可作为一项检测有否砷中毒及病变程度的指标。
Objective To detect the mutation frequency of peripheral blood red blood cell glycoprotein A (GPA) in patients with arsenic poisoning caused by high arsenic coal. Methods Peripheral blood erythrocytes were isolated and immobilized in 40 patients with arsenic poisoning caused by arsenic, and then combined with fluorescein labeled McAb, the frequency of GPA mutation was analyzed by flow cytometry. Results The frequency of GPA mutation in peripheral blood erythrocytes of 40 patients with arsenic poisoning caused by high arsenic coal was (21.23 ± 13.97) × 10-6, the frequency of NO mutation was (33.13 ± 25.72) × 10-6, the frequency of MM mutation was (110.90 ± 63.58) × 10-6. The mutation frequency of MO was (20.35 ± 21.26) × 10-6. The mutation frequency of GPA was significantly higher than that of normal population (P <0.01). In the tumor group, the mutation frequency of NN was (31.50 ± 16.13) × 10-6, the mutation frequency of NO was (54.50 ± 38.13) × 10-6, the mutation frequency of MM was (159.33 ± 66.22) × 10-6. The mutation frequency of MO was 45.16 ± 12.69) × 10-6, the mutation frequency of GPA was significantly higher than that of non-tumor group (P <0.01). Conclusion Arsenism can result in the mutation of GPA in human peripheral erythrocytes. Suggesting that the frequency of GPA mutation can be used as an indicator of arsenic poisoning and lesion severity.