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为了更好地了解水稻腈水解酶家族的功能,采用生物信息学的方法对水稻中的腈水解酶进行了一系列分析,结果发现水稻中共有8个编码腈水解酶的基因,虽然基因结构很不相同,但其所编码的腈水解酶都具有典型的碳氮水解酶结构域和保守的Glu-Lys-Cys催化三联体。通过EST搜索发现这些基因在水稻不同组织和不同胁迫条件下的表达情况存在较大的差异。此外,对水稻和拟南芥腈水解酶进行系统进化分析发现其分别处于5个不同的分支,表明它们的分化早于单子叶植物和双子叶植物的分化。研究结果为进一步研究该类蛋白的分子和生物学功能奠定了良好基础。
In order to better understand the function of rice nitrilase family, bioinformatics methods were used to analyze a series of nitrilases in rice. As a result, a total of eight genes encoding nitrilase were found in rice, Are not the same, but all their encoded nitrilases have a typical carbonitrilase domain and a conserved Glu-Lys-Cys catalytic triad. EST search found that these genes in different tissues of rice and different stress conditions there is a big difference. In addition, phylogenetic analysis of rice and Arabidopsis nitrilase showed that they were located in five different branches, respectively, indicating that their differentiation preceded the differentiation of monocotyledons and dicotyledons. The results laid a good foundation for further study on the molecular and biological functions of these proteins.