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研究大肠正常组织和癌组织的激光诱导自体荧光光谱特征 ,为大肠癌的早期诊断提供理论依据。方法 :采用 370nm激光诱导自体荧光检测分析系统采集 2 4例外科手术切下的大肠癌组织和正常组织的自体荧光光谱 ,分析两者的荧光光谱差异。结果 :采集了 40 0～ 70 0nm波长范围的自体荧光光谱 ,40 0～ 5 90nm正常组织的荧光强度高于癌组织 ,5 90～ 70 0nm癌组织的荧光强度高于正常组织。在波长 46 0nm、6 90nm有特征波峰 ,在 46 0nm正常组织和癌组织的特征峰强度分别为 1193± 10 1、782± 88,正常组织的峰强度明显高于癌组织 (P <0 .0 0 1) ;在 6 90nm特征峰强度分别为 5 5 6± 2 8、6 18± 2 9,癌组织的荧光强度显著高于正常组织 (P <0 .0 0 1)。不同组织学类型的大肠癌荧光强度无明显差别。结论 :正常组织和癌组织的自体荧光强度存在差异 ,不同组织学类型的大肠癌荧光强度无明显差别。
To study the characteristics of laser-induced autofluorescence of normal colorectal tissue and cancerous tissue, provide theoretical basis for the early diagnosis of colorectal cancer. METHODS: Autofluorescence spectra of colorectal cancer tissue and normal tissue were collected from 24 patients who underwent 370 nm laser-induced autofluorescence detection and analysis system. The differences in fluorescence spectra were analyzed. RESULTS: Autofluorescence spectra were collected in the wavelength range of 400 ~ 700 nm. The fluorescence intensity of normal tissues from 400 to 490 nm was higher than that of cancer tissues. The fluorescence intensity of 490-70 nm cancer tissues was higher than that of normal tissues. There were characteristic peaks at the wavelengths of 46 0 nm and 6 90 nm, and the characteristic peak intensities of normal tissues and cancer tissues at 460 nm were 1193±10 1 and 782±88, respectively. The peak intensity of normal tissues was significantly higher than that of cancer tissues (P < .0 0 1) ; The intensity of characteristic peaks at 6 90 nm were 5 5 6 ± 2 8 and 6 18 ± 2 9. The fluorescence intensity of cancer tissue was significantly higher than that of normal tissues (P < 0.01). There was no significant difference in the fluorescence intensity of colorectal cancer of different histological types. Conclusion: The autofluorescence intensities of normal tissues and cancer tissues are different. There is no significant difference in the fluorescence intensity of different histological types of colorectal cancer.