论文部分内容阅读
本实验用人骨髓细胞建立起贴壁细胞层,做第二次骨髓接种后,每周换液时取出全部细胞悬液,换入等量的无细胞新鲜生长培养液,观察悬浮细胞和GM-CFUc数量变化的规律。在这种培养条件下,造血干细胞的增殖与分化可维持7周。虽然悬浮细胞和GM-GFUc的数量在培养第二周后下降较快,但在培养七周内悬液中始终存在少量悬浮细胞和GM-CFUc。对部分培养同时测定了悬液与贴壁层中的GM-CFUc,在培养2～3周时贴壁层中GM-CFUc有增多趋势,到5～8周时贴壁层中就测不出GM-CFUc,此时悬浮的GM-CFUc也很少了。实验结果说明在人骨髓体外培养悬液中的造血干细胞(或祖细胞)来源于贴壁层,贴壁层是维持体外造血干细胞增殖与分化的基地。
In this experiment, adherent cells were established with human bone marrow cells. After the second bone marrow inoculation, the whole cell suspension was removed every week when the liquid was changed, and the same amount of cell-free fresh growth medium was added to observe the suspension cells and GM-CFUc The law of quantity changes. Under this culture condition, proliferation and differentiation of hematopoietic stem cells can be maintained for 7 weeks. Although the number of suspended cells and GM-GFUc decreased more rapidly after the second week of culture, there was always a small amount of suspension cells and GM-CFUc in the suspension for seven weeks. On the other hand, GM-CFUc in suspension and adherent layer was measured simultaneously with partial culture. There was an increasing trend of GM-CFUc in the adherent layer during 2 to 3 weeks of culture, and it was not detected in the adherent layer at 5 to 8 weeks GM-CFUc, GM-CFUc suspended at this time is also very small. The experimental results show that the hematopoietic stem cells (or progenitor cells) in human bone marrow culture suspension originate from the adherent layer, which is the base for maintaining the proliferation and differentiation of hematopoietic stem cells in vitro.