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采用固相微萃取与气相色谱-质谱联用技术,对油桃果实采后衰老与褐腐病害发生中近表皮和近果核的挥发性物质进行测定;用主成分分析(PCA)法研究不同样品挥发物谱的差异并对香气品质进行综合评价。结果表明,共检测到油桃果实挥发性物质14种,主要为C6化合物、酯类和内酯类物质。贮藏期间油桃果实挥发性物质逐渐由近果核处转向近表皮,褐腐病害发生后加速了这种转变。PCA结果表明,5-羟基-2,4-癸二烯酸-γ-内酯、γ-癸内酯、δ-辛内酯和乙醇可作为区分油桃果实贮藏4d时衰老与病害发生的标志性挥发物;C6醛类、乙醇和乙酸乙酯则可作为区分油桃果实贮藏0、2d与贮藏4、6d时的标志性挥发物。病害组贮藏2d后近表皮处香气品质最佳,贮藏4d后病害组香气品质远低于衰老组。
Solid phase microextraction coupled with gas chromatography-mass spectrometry (GC-MS) was used to determine the volatile substances in the near-epidermis and near-nucleus of postharvest nectarine fruit senescence and brown rot disease. Principal component analysis (PCA) Variations in sample volatiles and a comprehensive evaluation of aroma quality. The results showed that a total of 14 kinds of volatile substances were detected in nectarine fruit, mainly C6 compounds, esters and lactones. Volatile substances in nectarine fruits gradually shift from near fruit nuclei to near epidermis during storage, and this transformation accelerated after brown rot disease. PCA results showed that 5-hydroxy-2,4-decadienoyl-γ-lactone, γ-decalactone, δ-octanoic lactone and ethanol could be used as markers to distinguish the senescence and disease of nectarine stored for 4 days Volatile compounds; C6 aldehydes, ethanol and ethyl acetate can be used as distinguishing nectarine fruit storage 0,2 d and storage 4,6 d when the landmark volatile. After 2 days of storage, the aroma quality of the near epidermis was the best, and the aroma quality of the disease group was far lower than that of the aging group after 4 days of storage.