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目的:研究抗缺氧物质对小鼠抗缺氧能力的影响及Hsp70的表达。方法:小鼠100只,随机分为塞隆(高原鼢鼠)肌肉组、高海拔牦牛肉组、石榴籽组、红景天组和空白对照组,连续灌胃(ig)10天,于常压下进行抗缺氧实验和Western Blot测定Hsp70的表达。结果:红景天组、石榴籽组、塞隆肉组小鼠密闭缺氧存活时间延长;塞隆肉组、石榴籽组、红景天组灌胃的小鼠的Hsp70表达被诱导出现。结论:抗缺氧物质(即红景天胶囊、石榴籽超临界萃取物、塞隆肉超临界萃取物)灌胃的小鼠密闭缺氧存活时间均延长;抗缺氧物质灌胃的小鼠的Hsp70的表达增强,说明抗缺氧药物可诱导Hsp70的表达,Hsp或许可以作为抗缺氧药物研究和测试指标。
Objective: To study the effect of anti-hypoxia on hypoxia tolerance and Hsp70 expression in mice. Methods: One hundred mice were randomly divided into three groups: saron (plateau zokor) muscle group, high altitude yak meat group, pomegranate seed group, rhodiola group and blank control group. Depressed for anti-hypoxia experiments and Western Blot determination of Hsp70 expression. Results: Hypobaric survival time was prolonged in Rhodiola rosea, Pomegranate seed and Selene roux mice. Hsp70 expression was induced in the mice fed with Ceilong meat, Pomegranate seed and Rhodiola rosea. CONCLUSION: Mice with anti-hypoxic substances (ie, Rhodiola capsules, supercritical fluid extraction of pomegranate seeds and supercritical fluid of sarrionel) have longer duration of hypoxia, anti-hypoxic substance administered mice Hsp70 expression increased, indicating that anti-hypoxia drugs can induce the expression of Hsp70, Hsp may be used as anti-hypoxia drug research and testing indicators.