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目的探讨三丁基锡的肥胖激素效应。方法将幼龄非洲爪蟾(Xenopus laevis)置于浓度分别为50和100 ng/L的氯化三丁基锡溶液中暴露3个月,检测非洲爪蟾的体重、体长、躯干面积和脂肪重量等生长指标和肝脏组织的脂质变化。结果暴露1个月后,50 ng/L组体重比对照组增加了0.7倍,100 ng/L组体重、体长、躯干面积和脂肪重量比对照组分别增加了1.6、0.5、1.5和1.8倍;暴露2个月后,50 ng/L组躯干面积比对照组增加了0.5倍,100 ng/L组体重、体长、躯干面积和脂肪重量比对照组分别增加了0.6、0.2、0.7和2.8倍;暴露3个月后,100 ng/L组体长、躯干面积和脂肪重量比对照组分别增加了0.2、0.5和0.6倍。冰冻切片的脂质染色发现,暴露3个月,100 ng/L组中肝脏脂肪积累面积比对照组增加了1.7倍。结论TBT能够引起非洲爪蟾体重、体长、躯干面积和脂肪重量的增加,造成肝脏脂肪的积累,具有环境肥胖激素效应。鉴于非洲爪蟾在甲状腺激素和性激素等环境内分泌干扰物筛选中的广泛应用,可以考虑将其作为筛选环境肥胖激素的实验动物模型。
Objective To investigate the effects of tributyltin on the obesity hormone. Methods Young Xenopus laevis were exposed to tributyltin chloride solutions at concentrations of 50 and 100 ng / L for 3 months respectively. The body weight, body length, trunk area and fat mass of Xenopus laevis Growth Index and Lipid Changes in Liver Tissue. After 1 month of exposure, the body weight of 50 ng / L group increased 0.7 times than that of the control group. The body weight, body length, trunk area and fat weight of 100 ng / L group increased 1.6, 0.5, 1.5 and 1.8 times ; After 2 months of exposure, the trunk area of 50 ng / L group increased by 0.5 times than the control group, body weight, body length, trunk area and fat weight of 100 ng / L group increased 0.6, 0.2, 0.7 and 2.8 Fold; body length, trunk area and fat weight of 100 ng / L group increased by 0.2, 0.5 and 0.6 times respectively after three months of exposure compared with the control group. Lipid staining of frozen sections revealed that the area of liver fat accumulation in the 100 ng / L group increased by 1.7 times compared with the control group at 3 months of exposure. Conclusion TBT can cause body weight, body length, trunk area and fat weight of Xenopus laevis increased, resulting in the accumulation of hepatic fat with the effect of environmental hormonal hormone. Given the widespread use of Xenopus in environmental screening of endocrine disruptors such as thyroid hormones and sex hormones, it may be considered as an experimental animal model for the screening of environmental hormones.