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目的:观察饱和脂肪酸棕榈酸(Palmitate,PA)对小鼠MIN6β细胞生长的影响,从细胞周期角度来探讨其发生的分子机制。方法:采用5g/LBSA代替血清培养36h使MIN6细胞同步化处于G0期。然后用PA(0.25～1.0mmol/L,45min～24h)干预,采用MTT法检测细胞活力,流式细胞术测定细胞周期,采用Westenblot检测细胞周期相关蛋白CDK4、CyclinD1表达水平的变化。结果:和对照组相比(1)不同浓度PA均显著抑制MIN6细胞增殖(P<0.01);(2)PA亦能明显抑制细胞周期进程,使MIN6细胞周期更多滞留在G0/G1期(P<0.01),G2/M期与S期细胞比例降低(P<0.01);(3)PA能显著的抑制细胞周期相关蛋白CDK-4、Cy-clinD1的表达(P<0.05),并与细胞周期延迟一致。结论:PA抑制MIN6β细胞生长可能是通过降低MIN6β细胞的细胞周期相关蛋白cyclinD1/CDK-4的表达,导致从G1-S期的阻滞,从而减弱细胞增殖。
Objective: To observe the effect of saturated fatty acid palmitate (PA) on the growth of mouse MIN6β cells and to explore its molecular mechanism from the cell cycle perspective. METHODS: MIN6 cells were synchronized at G0 with 5 g / L BSA instead of serum for 36 h. Then the cells were treated with PA (0.25-1.0mmol / L, 45min-24h). The cell viability was measured by MTT assay. The cell cycle was determined by flow cytometry. The expression of CDK4 and CyclinD1 was detected by Westenblot. Results: Compared with the control group, the proliferation of MIN6 cells was significantly inhibited by different concentrations of PA (P <0.01); (2) PA also significantly inhibited the cell cycle progression, leaving more MIN6 cell cycle in the G0 / G1 phase P <0.01). The proportion of cells in G2 / M phase and S phase decreased (P <0.01). (3) PA could significantly inhibit the expression of CDK-4 and Cy- clinD1 (P < Cell cycle delay consistent. CONCLUSION: PA inhibits the growth of MIN6β cells by decreasing the expression of cyclinD1 / CDK-4, a cell cycle-related protein, in MIN6β cells, leading to a block from the G1-S phase, thereby attenuating cell proliferation.