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目的:对垂丝海棠花和叶α-葡萄糖苷酶抑制活性进行研究。方法:利用体外α-葡萄糖苷酶抑制模型,以阿卡波糖为阳性对照对α-葡萄糖苷酶抑制活性评价。结果:垂丝海棠花和叶的不同提取部位均具有一定的α-葡萄糖苷酶抑制活性,垂丝海棠叶70%乙醇部位(IC50=69.68 mg·L-1)的α-葡萄糖苷酶的抑制活性最好,远高于阳性对照阿卡波糖(IC50=1 213.38 mg·L-1)。垂丝海棠叶中70%乙醇部位、石油醚部位和乙酸乙酯部位活性均高于垂丝海棠花中相应部位,而叶中正丁醇部位略低于花的正丁醇部位,且都高于阳性对照阿卡波糖。此外,各部位的抑制活性均与质量浓度呈现相关性,具有一定的浓度依赖性。结论:垂丝海棠花和叶不同提取部位均具有一定的α-葡萄糖苷酶抑制活性,但不同提取部位其抑制活性有差别。
Objective: To study the inhibitory activity of α-glucosidase in flower and leaf of Begonia villosorha. Methods: Inhibition of α-glucosidase was evaluated by in vitro α-glucosidase inhibition model and acarbose as positive control. Results: The inhibitory activity of α-glucosidase in the different parts of the flowers and leaves of B. przewalskii was inhibited. The inhibitory effect of α-glucosidase at 70% ethanol (IC50 = 69.68 mg · L-1) The activity was the best, far higher than the positive control acarbose (IC50 = 1 213.38 mg · L -1). The activity of 70% ethanol fraction, petroleum ether fraction and ethyl acetate fraction in the leaves of B. princetonii was higher than that of the corresponding fractions of the flowers, whereas the n-butanol fraction in the leaves was slightly lower than the n-butanol fraction in the flowers, Positive control acarbose. In addition, the inhibitory activity of each site showed a correlation with the mass concentration, with a certain concentration-dependent. CONCLUSION: All of the extracts from flowers and leaves of B. przewalskii possess certain inhibitory activity on α-glucosidase, but the inhibitory activity differs in different extracts.