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目的 培养、建立稳定的兔肝 ＶＸ－２移植癌模型，探讨不同植瘤方式的成功率。方法 ６０只新西兰白兔随机分 ３组，每组 ２０只。分别以不同的方式将 Ｖｘ－２瘤细胞植入兔的肝脏。观察：１．不同方式植瘤的成活率；２．Ｂ超测定肝内肿瘤 ７ｄ、１０ｄ、１４ｄ、１７ｄ、２１ｄ时的大小，并计算肿瘤生长率；３．大体及镜下（光镜和电镜）瘤组织形态特征。结果 １．三组植瘤成活率分别为 ７／２０、１０／２０、１９／２０，改良组植瘤成活率最高（Ｐ＜０．０５），瘤体呈指数性生长；２．病理学及ＣＴ表明该瘤在肝组织中呈浸润式生长，其性状与移植于兔其它部位的Ｖｘ－２鳞状细胞癌特征相似。结论 成功建立了兔肝Ｖｘ－２移植癌模型，瘤组织块种植方式成功率明显高于其它两组方式，为肝癌介入治疗的基础及临床研究，提供了成熟的大型实验动物模型。
Objective To culture and establish a stable rabbit VX-2 transplanted carcinoma model to explore the success rate of different tumor types. Methods Sixty New Zealand white rabbits were randomly divided into 3 groups (20 in each group). Vx-2 tumor cells were implanted into rabbit liver in different ways. Observation: 1. Different ways of survival rate of tumor; 2. B ultrasound measurement of intrahepatic tumors 7d, 10d, 14d, 17d, 21d size, and calculate the rate of tumor growth; 3. Gross and microscopic (light and electron microscopy) tumor morphological features. Results 1. The survival rates of the three groups were 7/20, 10/20 and 19/20, respectively. The survival rate of the modified group was the highest (P <0.05), and the tumor grew exponentially. 2. Pathology and CT showed that the tumor grew infiltratively in liver tissue with similar characteristics to Vx-2 squamous cell carcinoma transplanted in other parts of the rabbit. Conclusion The successful establishment of rabbit Vx-2 transplanted carcinoma model has a significantly higher successful rate of tumor mass implantation compared with the other two groups. It provides a mature large experimental animal model for the basic and clinical study of interventional therapy of liver cancer.