准确定量检测血清中HBV-DNA能反映HBV在肝脏复制的程度,对乙肝治疗监测具有极其重要的意义,同时也是考核抗病毒药物治疗效果的重要依据。PCR法检测HBV-DNA成为最常用的检测手段,由于PCR法比较精细,可受一系列的技术及人为因素的影响,因此检测结果易产生误差,甚至造成假阴性。为 Accurate and quantitative detection of serum HBV-DNA can reflect the extent of HBV replication in the liver, the treatment of hepatitis B has an extremely important significance, but also an important basis for the evaluation of antiviral drug treatment. PCR-based detection of HBV-DNA has become the most commonly used detection means. As the PCR method is more sophisticated, it can be affected by a series of technical and human factors, so the test results are prone to error and even false negative. for