目的:明确甘草在精子发生过程中对精原细胞增殖的影响。方法:选取3只健康6日龄SPF级雄性C57BL/6J小鼠,采用小鼠睾丸器官培养方式进行抗-增殖细胞核抗原(PCNA)-抗体的免疫组化染色和细胞增殖活性检测,在光学显微镜下观察精原细胞中的PCNA阳性细胞率,考察增殖活性。结果:与对照组相比,甘草组明显促进精原细胞的增殖,且呈浓度依赖性。结论:在小鼠精子发生过程中,甘草能够有效地促进精原细胞的增殖。 Objective: To clarify the licorice spermatogenesis in the process of spermatogonial proliferation. Methods: Three healthy 6-day-old male C57BL / 6J mice were used to detect the expression of anti-proliferating cell nuclear antigen (PCNA) -antibodies in mouse testis organ culture and the cell proliferation activity was detected by light microscopy Under the observation of PCNA-positive cells in spermatogonia, the proliferative activity was examined. Results: Compared with the control group, licorice root group significantly promoted the proliferation of spermatogonia in a concentration-dependent manner. Conclusion: Licorice can effectively promote the proliferation of spermatogonia during mouse spermatogenesis.