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为研究大鼠垂体黄体生成素的α和β亚基的生物合成及分泌,首先需要得到高纯度的促性腺细胞。继往,人们曾使用密度梯度离心法来分离不同的垂体细胞,但其细胞存活率和回收率都比较低。我们使用离心分选技术来处理经酶解的完整大鼠垂体细胞,虽细胞成活率及回收率明显提高,但并不能将促性腺细胞与促甲状腺素分泌细胞适当分开。然而应用阉割30天的雄性大鼠的垂体进行实验,经离心分选后,可得到相对纯净的促性腺细胞群,并可使之与促甲状腺素分泌细胞相对分开,离体培养条件下,加入促性腺激素释放激素,可以刺激较纯净的促性腺细胞分泌LH增加。因此我们认为用离心分选技术所得之相对纯净的促性腺细胞可以作为研究大鼠LH亚基合成及分泌的离体模型。
In order to study the biosynthesis and secretion of α and β subunits of rat pituitary lutein, it is first necessary to obtain high-purity gonadal cells. In the past, people have used density gradient centrifugation to separate different pituitary cells, but their cell viability and recovery rates are relatively low. We use centrifugation techniques to treat the intact rat pituitary cells, although the viability and recovery rate of the cells are significantly increased, but we can not properly divide the gonadotrophs and TSH-secreting cells. However, the application of castration 30-day-old male rats pituitary experiments, after centrifugation, you can get a relatively pure group of gonadotropic cells, and make it relatively separated from thyrotropin-secreting cells, cultured in vitro conditions, adding Gonadotropin-releasing hormone, can stimulate more pure gonadal secretion of LH increased. Therefore, we believe that relatively pure gonadotrophic cells obtained by centrifugation can be used as ex vivo models to study the synthesis and secretion of LH subunits in rats.