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本文研究Mg~(2+)对H~+-ATP酶在脂酶体中慢运动的影响。在不同Mg~(2+)浓度及不同温度下,测量了运用保温法嵌入脂酶体中的马来酰亚胺自旋标记的H~+-ATP酶的ST-EPR谱。由ST-EPR谱的诊断参数L″/L,C′/C与Mg~(2+)浓度以及温度的关系,并对照根据相同条件下测得的5NS-EPR谱超精细分裂而得到的序参数S与Mg~(2+)浓度以及温度的关系,分析得出:增加脂酶体中的Mg~(2+)浓度与降低测量温度都导致H~+-ATP酶在膜脂中的运动相关时间增加。进一步分析还得到H~+-ATP酶在膜脂中旋转扩散运动的快慢与周围膜脂的活化能大小有关。在脂酶体中Mg~(2+)浓度的变化将导致膜脂活化能发生改变,从而影响H~+-ATP酶在膜中的慢运动状态。
In this paper, the effect of Mg 2+ on the slow motility of H + -ATPase in liposomes was studied. The ST-EPR spectra of maleimide spin-labeled H ~ + -ATPase embedded in liposomes by thermal incubation method were measured at different Mg 2+ concentrations and different temperatures. According to the relationship between the diagnostic parameters L "/ L, C ’/ C and Mg 2+ concentration and temperature of the ST-EPR spectrum, and according to the hyperfine division of the 5NS-EPR spectrum measured under the same conditions The results showed that increasing the concentration of Mg 2+ in the liposome and decreasing the measuring temperature all resulted in the movement of H + -ATPase in the membrane lipids Related time increase.Further analysis also got H ~ + -ATP enzyme in the membrane lipid spin diffusion movement speed and the surrounding membrane lipid activation energy size in the changes in the concentration of Mg ~ (2 +) in the liposome will lead to membrane Lipid activation energy changes, thus affecting the H ~ + -ATP enzyme in the membrane slow motion.