论文部分内容阅读
为了明确结球甘蓝无蜡粉亮绿突变体材料LD10的特征特性及遗传规律,测定了LD10的主要农艺性状和营养物质含量;对LD10和普通材料21-3的叶面超微结构进行了观察比较;以LD10和21-3为亲本构建6世代群体,对LD10无蜡粉亮绿性状遗传规律进行分析。结果表明:无蜡粉亮绿突变体材料LD10叶面蜡粉呈小颗粒状结构,缺失严重,而普通材料21-3叶面蜡粉呈长棒状结构,含量丰富,二者具有明显差异;LD10的无蜡粉亮绿性状由1个隐性基因(cgl-4)控制,其与SSR标记LT-SSR16紧密连锁,遗传距离为4.7c M。用LD10与另一单基因隐性遗传的无蜡粉亮绿突变体材料10Q-961杂交获得F1群体,F1群体单株全部表现为叶面有蜡粉,推断LD10与10Q-961的无蜡粉亮绿基因位于不同的遗传位点。
In order to clarify the characteristics and inheritance of LD10, a wax-free bright green mutant of cabbage, the main agronomic traits and nutrient contents of LD10 were determined. The leaf ultrastructure of LD10 and common material 21-3 were observed Six generations of LD10 and 21-3 were used as parents to analyze the genetic rule of LD10 without wax and bright green traits. The results showed that the waxy green mutant LD10 leaves showed a small granular structure with a serious lack of wax, whereas the common material 21-3 leaf wax showed a long rod-shaped structure with abundant content, with significant differences between the two. LD10 The wax-free bright green trait was controlled by a recessive gene (cgl-4), which was closely linked to the SSR marker LT-SSR16 with a genetic distance of 4.7cM. The F1 population was obtained by crossing LD10 with another unigenes mutant waxless bright green mutant 10Q-961. All the F1 population showed the presence of wax powder on the foliage, and deduced that LD10 and 10Q-961 wax-free powder Brilliant green genes are located at different genetic loci.