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目的：探讨氯乙醇（ＣＥ）对肝线粒体钙泵的影响及其钙泵抑制的机制。方法：ＣＥ与肝线粒体共同温浴，分别测定丙二醛（ＭＤＡ）、蛋白巯基及钙泵的变化。结果：ＣＥ能引起线粒体的脂质过氧化（ＬＰＯ），表现为ＭＤＡ升高，同时，还引起蛋白巯基的降低和钙泵的抑制，与对照组比较具有显著性意义，且存在着剂量反应关系及时间反应关系。在本实验条件下，发现ＣＥ引起的蛋白巯基的降低与钙泵抑制呈平衡关系。用巯基供剂二硫苏糖醇（ＤＴＴ）能部分地拮抗ＣＥ引起肝线粒体ＬＰＯ，保护了线粒体蛋白巯基，从而保护了钙泵。结论：钙泵的抑制可能与ＬＰＯ耗竭蛋白巯基有关。
Objective: To investigate the effect of chloroethanol (CE) on hepatic mitochondrial calcium pump and its mechanism of calcium pump inhibition. Methods: CE and liver mitochondria were incubated with different concentrations of malondialdehyde (MDA), protein sulfhydryl and calcium pump. Results: CE could induce lipid peroxidation (LPO) in mitochondria, which showed the increase of MDA. At the same time, it also caused the reduction of protein sulfhydryl and inhibition of calcium pump, which was significant compared with the control group, and there was dose-response Relationship and time response relationship. Under the experimental conditions, it was found that the decrease of protein thiol induced by CE was in equilibrium with the inhibition of calcium pump. Dithiothreitol (DTT), a thiol-donating agent, is able to partially antagonize CE-induced hepatic mitochondrial LPO and protect the mitochondrial protein thiol, thus protecting the calcium pump. Conclusion: The inhibition of calcium pump may be related to LPO depletion of sulfhydryl groups.