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通过测定 4 5Ca2 +摄取初速度的变化研究了La3+和Gd3+对鼠肝癌H 3 5细胞Ca2 +内流的影响 .发现低浓度La3+和Gd3+能增加肝癌H 3 5细胞Ca2 +内流初速度达 6～ 10倍 .Ca2 +内流的初速度随Ca2 +浓度变化的动力学研究表明 ,肝癌H 3 5细胞中存在 2类Ca2 +亲和部位的通道 ,即高亲和位和低亲和位Ca2 +内流通道 ,而La3+和Gd3+刺激的肝癌H 3 5细胞则只有 1类Ca2 +亲和位通道 ,La3+和Gd3+增加高亲和位Ca2 +结合通道的Km和Vmax,而对于低亲和位的Ca2 +结合通道Ca2 +内流的作用是竞争性的 .
The effects of La3+ and Gd3+ on Ca2 + influx in murine hepatocarcinoma H3 5 cells were studied by measuring the initial velocity of 45Ca2 + uptake. It was found that low concentrations of La3+ and Gd3+ can increase the initial velocity of Ca2 + influx in H3 5 cells of hepatoma up to 6 The kinetics of the initial velocity of Ca2+ influx with the concentration of Ca2 + in the Ca2+ influx demonstrated that there are two types of Ca2 + affinity site channels, ie high and low affinity Ca2, in H3S cells. + Influx channels, while La3+ and Gd3+ stimulated hepatoma H3 cells have only one type of Ca2+ affinity channel, La3+ and Gd3+ increase the Km and Vmax of high affinity Ca2+ binding channels, and for low affinity sites The role of Ca2+ in Ca2 + influx is competitive.