With respect to the ergonomic evaluation and optimization in the mental task design of the aircraft cockpit display interface, the experimental measurement and theoretical modeling of mental workload were carried out under flight simulation task conditions using the performance evaluation, subjective evaluation and physiological measurement methods. The experimental results show that with an increased mental workload, the detection accuracy of flight operation significantly reduced and the reaction time was significantly prolonged; the standard deviation of R-R intervals(SDNN) significantly decreased, while the mean heart rate exhibited little change; the score of NASA_TLX scale significantly increased. On this basis, the indexes sensitive to mental workload were screened, and an integrated model for the discrimination and prediction of mental workload of aircraft cockpit display interface was established based on the Bayesian Fisher discrimination and classification method. The original validation and cross-validation methods were employed to test the accuracy of the results of discrimination and prediction of the integrated model, and the average prediction accuracies determined by these two methods are both higher than 85%. Meanwhile, the integrated model shows a higher accuracy in discrimination and prediction of mental workload compared with single indexes. The model proposed in this paper exhibits a satisfactory coincidence with the measured data and could accurately reflect the variation characteristics of the mental workload of aircraft cockpit display interface, thus providing a basis for the ergonomic evaluation and optimization design of the aircraft cockpit display interface in the future. With respect to the ergonomic evaluation and optimization in the mental task design of the aircraft cockpit display interface, the experimental measurement and theoretical modeling of mental workload were carried out under flight simulation task conditions using the performance evaluation, subjective evaluation and physiological measurement methods. experimental results show that with an increased mental workload, the detection accuracy of flight operation significantly reduced and the reaction time was significantly prolonged; the standard deviation of RR intervals (SDNN) significantly decreased, while the mean heart rate showed little change; the score of NASA_TLX scale significantly increased. On this basis, the indexes sensitive to mental workload were screened, and an integrated model for the discrimination and prediction of mental workload of aircraft cockpit display interface was established based on the Bayesian Fisher discrimination and classification method. The origin al validation and cross-validation methods were employed to test the accuracy of the results of discrimination and prediction of the integrated model, and the average prediction accuracies determined by these two methods are both higher than 85%. Meanwhile, the integrated model shows a higher accuracy in discrimination and prediction of mental workload compared with single indexes. The model proposed in this paper exhibits a satisfactory coincidence with the measured data and could not reflect the variation characteristics of the mental workload of aircraft cockpit display interface, thus providing a basis for the ergonomic evaluation and optimization design of the aircraft cockpit display interface in the future.