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目的 探讨中药钙离子通道拮抗剂人参皂甙单体Rb1对白血病多药耐药细胞系 (K562 /HHT)细胞内柔红霉素浓度的影响。方法 细胞内柔红霉素浓度荧光测定法、细胞P GP表达常规APAAP测定法、mdr1基因表达RT PCR测定法等实验方法 ,探讨Rb1的逆转机理。结果 Rb1作用后K562 /HHT细胞内DNR浓度增加 (P <0 .0 1 )。结论 K562 /HHT是一种以P GP介导的多药耐药细胞系 ,检测显示K562 /HHTP GP表达阳性率 1 0 0 % ,并有很强的mdr1基因表达 ,提示Rb1通过抑制P GP外排药物功能 ,提高细胞内药物浓度 ,是Rb1逆转耐药作用的主要机理。
Objective To investigate the effects of ginsenoside Rb1, a calcium channel antagonist of traditional Chinese medicine, on the concentration of daunorubicin in the leukemia multidrug resistant cell line (K562/HHT). Methods The intracellular erythromycin concentration fluorescence assay, cell P GP expression routine APAAP assay, and mdr1 gene expression RT PCR assay were used to explore the mechanism of Rb1 reversal. Results The concentration of DNR in K562/HHT cells increased after Rb1 treatment (P < 0.01). Conclusions K562/HHT is a multidrug resistant cell line mediated by PGP. The detection rate of K562/HHTP GP expression is 100%, and there is a strong mdr1 gene expression, suggesting that Rb1 inhibits GP by Rowing drug function and increasing intracellular drug concentration are the main mechanisms of Rb1 reversal of drug resistance.